Aspects of Standardization in the Juice of the Herbs <i>Alchemilla vulgaris</i>

Introduction. The features of the chemical composition and identified pharmacological activities substances from fresh raw materials Alchemilla vulgaris allow us to predict prospects for use juice herb in medical practice. creation drugs based on juices involves development methods their standardization. Aim. To determine qualitative analysis identification quantitative assessment main group biologically active juice. Materials methods. production was harvested during flowering period various habitats. phenolic compounds studied by paper chromatography thin layer (TLC). determination procedure developed validated an SF-2000 spectrophotometer. Results discussion. Paper (CP) revealed derivatives cinnamic phenolcarboxylic acids, a flavonoids with predominance flavones flavonols, rutin among dominant ones. TLC butanol : acetic acid water system (4 1 2), we achieved optimal separation rutin, which proposed as substance Correspondence configuration differential spectrum complex cuff aluminum chloride similar SS results give grounds standard. best option when choosing aliquot reliable showed 0.2 ml content samples ranged 2.15 ± 0.24 % 7.34 0.16 %. Conclusion. butanol-acetic acid-water 2) can be used authenticate . parameters method are taking into account variation ratio component dry residue is valid standardize taxation variability depending growing conditions.